对喂食致动脉粥样硬化饮食的纯种狒狒血清中高密度脂蛋白（HDL）以及低密度脂蛋白（LDL）与HDL之间的脂蛋白中间体的表征。

Characterization of HDL and lipoproteins intermediate to LDL and HDL in the serum of pedigreed baboons fed an atherogenic diet.

作者信息

Babiak J, Gong E L, Nichols A V, Forte T M, Kuehl T J, McGill H C

出版信息

Atherosclerosis. 1984 Jul;52(1):27-45. doi: 10.1016/0021-9150(84)90154-0.

Abstract

A lipoprotein species with ultracentrifugal flotation rates (F0(1.20) 9-28) intermediate to high density lipoproteins (HDL, F0(1.20) 0-9) and low density lipoproteins (LDL, F0(1.20) 28-56) found in the plasma of certain pedigreed baboons fed an atherogenic diet was studied by gradient gel electrophoresis (GGE) and ultracentrifugal techniques. These lipoproteins were found to be heterogeneous in size (125-220 A) and hydrated density (1.028-1.080 g/ml). The major apolipoprotein in all density subfractions of the F0(1.20) 9-28 lipoproteins exhibited the molecular weight (2.8 X 10(4) daltons) and immunochemical properties of apolipoprotein A-I (apoA-I). Protein corresponding to apolipoprotein E (apoE, 3.5 X 10(4) daltons) was observed primarily in the less dense subspecies of F0(1.20) 9-28 lipoproteins. Some low molecular weight (1.8 X 10(4), 1.3 X 10(4), and 1.1 X 10(4) daltons) apolipoproteins were also detected. At low serum F0(1.20) 9-28 lipoprotein concentrations, only the smaller, more dense, protein-rich species were present; at higher F0(1.20) 9-28 concentrations, the larger, less dense species were observed in addition to the small species. The HDL of pedigreed baboons in families with and without serum F0(1.20) 9-28 lipoproteins were also characterized. The HDL of both groups of progeny consisted of a similar set of 5 subpopulations designated HDL-I through HDL-V determined by GGE. HDL-I, consisting of material 100-125 A in size, was the major HDL subpopulation. ApoA-I was the major protein moiety in all HDL subpopulations; none contained apoE. Baboons in families with F0(1.20) 9-28 lipoproteins had more HLD-I (292 +/- 80 mg/dl vs. 235 +/- 55 mg/dl) and less HDL-II (86 +/- 22 mg/dl vs. 135 +/- 34 mg/dl) than baboons in families without F0(1.20) 9-28 lipoproteins; both groups showed identical total HDL concentrations (446 +/- 90 mg/dl and 444 +/- 49 mg/dl, respectively). Among those baboons in families with F0(1.20) 9-28 lipoproteins, there was an inverse correlation between F0(1.20) 9-28 concentration and total HDL, HDL-I and HDL-II concentrations, indicating a possible metabolic relationship between these HDL subpopulations and the F0(1.20) 9-28 species.

摘要

通过梯度凝胶电泳（GGE）和超速离心技术，对特定纯种狒狒喂食致动脉粥样化饮食后血浆中发现的一种脂蛋白进行了研究。该脂蛋白的超速离心漂浮率（F0(1.20) 9 - 28）介于高密度脂蛋白（HDL，F0(1.20) 0 - 9）和低密度脂蛋白（LDL，F0(1.20) 28 - 56）之间。这些脂蛋白在大小（125 - 220 Å）和水合密度（1.028 - 1.080 g/ml）方面存在异质性。F0(1.20) 9 - 28脂蛋白所有密度亚组分中的主要载脂蛋白表现出载脂蛋白A - I（apoA - I）的分子量（2.8×10⁴道尔顿）和免疫化学特性。对应于载脂蛋白E（apoE，3.5×10⁴道尔顿）的蛋白质主要在F0(1.20) 9 - 28脂蛋白密度较低的亚组分中观察到。还检测到一些低分子量（1.8×10⁴、1.3×10⁴和1.1×10⁴道尔顿）的载脂蛋白。在低血清F0(1.20) 9 - 28脂蛋白浓度时，仅存在较小、密度更高、富含蛋白质的种类；在较高的F0(1.20) 9 - 28浓度时，除了小种类外还观察到较大、密度较低的种类。对有和无血清F0(1.20) 9 - 28脂蛋白的家族中纯种狒狒的HDL也进行了表征。两组后代的HDL均由通过GGE确定的一组类似的5个亚群组成，命名为HDL - I至HDL - V。HDL - I由大小为100 - 125 Å的物质组成，是主要的HDL亚群。ApoA - I是所有HDL亚群中的主要蛋白质部分；均不含apoE。有F0(1.20) 9 - 28脂蛋白的家族中的狒狒比没有F0(1.20) 9 - 28脂蛋白的家族中的狒狒具有更多的HLD - I（292±80 mg/dl对235±55 mg/dl）和更少的HDL - II（86±22 mg/dl对135±34 mg/dl）；两组的总HDL浓度相同（分别为446±90 mg/dl和444±49 mg/dl）。在有F0(1.20) 9 - 28脂蛋白的家族中的那些狒狒中，F0(1.20) 9 - 28浓度与总HDL、HDL - I和HDL - II浓度之间存在负相关，表明这些HDL亚群与F0(1.20) 9 - 28种类之间可能存在代谢关系。