Henderson T J, Bigbee J W, DeVries G H
Brain Res. 1984 Jul 23;306(1-2):179-88. doi: 10.1016/0006-8993(84)90367-6.
Bovine myelin-free axonal preparations were subjected to a series of washes designed to partition membranes from other cellular components initially present in these preparations. These washes were composed entirely of membranous structures, essentially free of neurofilament protein subunits, and contained high specific activity of acetylcholinesterase, an axolemma-specific enzyme. The distribution of acetylcholinesterase in the washes paralleled the distribution of lipid and the lipid composition of these washes closely resembled that of bovine axolemma-enriched fractions. In addition, acetylcholinesterase, lipid and galactocerebroside were histo- and immunohistochemically localized on similar structures in the starting material. Our results demonstrate that some of the lipid in myelin-free axonal preparations may be accounted for by axolemma.
对无髓鞘牛轴突制剂进行了一系列洗涤，目的是将膜与这些制剂中最初存在的其他细胞成分分开。这些洗涤液完全由膜结构组成，基本不含神经丝蛋白亚基，并含有高比活性的乙酰胆碱酯酶，这是一种轴膜特异性酶。洗涤液中乙酰胆碱酯酶的分布与脂质的分布平行，且这些洗涤液的脂质组成与富含牛轴膜的组分非常相似。此外，乙酰胆碱酯酶、脂质和半乳糖脑苷脂在起始材料中的相似结构上进行了组织化学和免疫组织化学定位。我们的结果表明，无髓鞘轴突制剂中的一些脂质可能来自轴膜。
