Lindvall O, Björklund A, Skagerberg G
Brain Res. 1984 Jul 23;306(1-2):19-30. doi: 10.1016/0006-8993(84)90352-4.
The distribution of dopamine (DA)-containing fibers in the virtual absence of noradrenaline (NA)-containing ones has been mapped by aldehyde fluorescence histochemistry in rats subjected to a combined neurotoxin treatment (intracerebral 6-hydroxydopamine injections plus systemic injections of the selective NA neurotoxin DSP-4). This pretreatment left di- and telencephalic DA levels largely unaffected, but reduced the NA levels by at least 86-96%. The resulting DA:NA ratios suggested that the catecholamine-containing structures, demonstrable by fluorescence histochemistry in the di- and telencephalic regions, were predominantly the DA-containing ones. While the distribution of DA terminal systems in the neo- and allocortical regions conformed well to previous results, the combined neurotoxin treatment revealed new features of the distribution of DA fibers in the diencephalon. In addition to the previously described innervations of the tubero-hypophyseal system, the incerto-hypothalamic system, and the mesohabenular pathway, previously unknown innervations were revealed in the supraoptic, paraventricular and dorsomedial nuclei of the hypothalamus, and in the paraventricular nucleus of the thalamus. Apart from some scattered fibers in the periventricular and lateral hypothalamic areas and medical zona incerta, other diencephalic nuclei seemed to be devoid of any significant DA terminal networks. The dopaminergic nature of these innervations is supported by DA uptake experiments (evaluated by fluorescence histochemistry) as well as by independent biochemical and immunohistochemical evidence. It is suggested that the DA innervations of the hypothalamic neurosecretory nuclei originate in cell bodies of the diencephalic A11-A14 cell groups and that such intradiencephalic DA projections participate in the regulation of oxytocin and vasopressin release from the pituitary.
采用醛荧光组织化学方法，在接受联合神经毒素处理（脑室内注射6-羟基多巴胺加全身注射选择性去甲肾上腺素神经毒素DSP-4）的大鼠中，绘制了几乎不存在含去甲肾上腺素（NA）纤维情况下含多巴胺（DA）纤维的分布图。这种预处理使间脑和端脑的DA水平基本未受影响，但使NA水平降低了至少86%-96%。由此产生的DA:NA比值表明，通过荧光组织化学在间脑和端脑区域可显示的含儿茶酚胺结构主要是含DA的结构。虽然新皮质和旧皮质区域DA终末系统的分布与先前结果相符，但联合神经毒素处理揭示了间脑DA纤维分布的新特征。除了先前描述的对结节-垂体系统、不确定-下丘脑系统和中缰通路的神经支配外，还发现了下丘脑视上核、室旁核和背内侧核以及丘脑室旁核中以前未知的神经支配。除了在室周和下丘脑外侧区以及内侧未定带中有一些散在纤维外，其他间脑核似乎没有任何明显的DA终末网络。这些神经支配的多巴胺能性质得到了DA摄取实验（通过荧光组织化学评估）以及独立的生化和免疫组织化学证据的支持。有人提出，下丘脑神经分泌核团的DA神经支配起源于间脑A11-A14细胞群的细胞体，并且这种间脑内的DA投射参与调节垂体后叶催产素和加压素的释放。
