Manns M, Meyer zum Büschenfelde K H, Slusarczyk J, Dienes H P
Clin Exp Immunol. 1984 Sep;57(3):600-8.
A radioimmunoassay (RIA) was developed for the detection of liver-kidney microsomal (LKM) autoantibodies. These were detected in four of 62 patients with HBsAg negative chronic active hepatitis (CAH) and in one patient with mixed connective tissue disease (MCTD). LKM antibodies were not detected in other hepatic and non-hepatic diseases. Other autoantibodies, especially anti-mitochondrial ones, do not react in this assay system. Sera positive for LKM antibodies by RIA showed a cytoplasmic staining of hepatocytes and proximal renal tubules by immunofluorescence. The LKM antigen was detected by RIA in microsomes prepared from rat liver, kidney, stomach, heart, lung, and skeletal muscle. It was destroyed after treatment with trypsin and chymotrypsin, but preserved after treatment with RNAase, DNAase and neuraminidase. Upon centrifugation of purified rat liver microsomes in CsCl gradient, LKM reactivity was detected at a density of 1.20 g/ml. In addition, the M2 antigen of the inner mitochondrial membrane specific for primary biliary cirrhosis (PBC) was localized at 1.28 g/ml in these density gradient fractions. The LKM antigen could not be solubilized. The presence of LKM antibodies characterizes a distinct subgroup of HBsAg negative CAH; they do not occur in PBC.
已开发出一种放射免疫分析法（RIA）用于检测肝肾微粒体（LKM）自身抗体。在62例乙肝表面抗原（HBsAg）阴性的慢性活动性肝炎（CAH）患者中有4例检测到了这些抗体，在1例混合性结缔组织病（MCTD）患者中也检测到了。在其他肝脏和非肝脏疾病中未检测到LKM抗体。其他自身抗体，尤其是抗线粒体抗体，在该检测系统中无反应。通过RIA检测LKM抗体呈阳性的血清，经免疫荧光检测显示肝细胞和近端肾小管有细胞质染色。通过RIA在从大鼠肝脏、肾脏、胃、心脏、肺和骨骼肌制备的微粒体中检测到了LKM抗原。用胰蛋白酶和糜蛋白酶处理后该抗原被破坏，但用核糖核酸酶、脱氧核糖核酸酶和神经氨酸酶处理后仍保留。将纯化的大鼠肝脏微粒体在氯化铯梯度中离心后，在密度为1.20 g/ml处检测到LKM反应性。此外，原发性胆汁性肝硬化（PBC）特有的线粒体内膜M2抗原在这些密度梯度级分中的定位为1.28 g/ml。LKM抗原无法溶解。LKM抗体的存在是HBsAg阴性CAH的一个独特亚组的特征；它们在PBC中不出现。
