测定氨基丙基转移酶的快速简便方法。
Rapid and convenient method for the assay of aminopropyltransferases.
作者信息
Hibasami H, Pegg A E
出版信息
Biochem J. 1978 Mar 1;169(3):709-12. doi: 10.1042/bj1690709.
Abstract
A new method for the assay of aminopropyltransferase activity is described. The method measures the formation of [methyl-14C]methylthioadenosine from decarboxylated S-adenosyl[methyl-14C]methionine in the presence of an amine acceptor. When used with extracts from rat ventral prostate, kidney, liver or brain, and with putrescine or spermidine as amines, the method gave results in excellent agreement with those obtained by the much more time-consuming conventional method. It was found that 1,3-diamino-propane and 1,8-diamino-octane were not acceptors for the prostatic enzyme fraction, but 1,5-diaminopentane (cadaverine) was active and 1,9-diaminononane and 1,12-diaminododecane also lead to the production of [methyl-14C]methylthioadenosine.
摘要
本文描述了一种测定氨丙基转移酶活性的新方法。该方法在胺受体存在的情况下，测量由脱羧的S-腺苷[甲基-¹⁴C]甲硫氨酸生成[甲基-¹⁴C]甲硫基腺苷的过程。当将该方法用于大鼠腹侧前列腺、肾脏、肝脏或脑的提取物，并以腐胺或亚精胺作为胺时，所得结果与耗时长得多的传统方法所获结果高度一致。研究发现，1,3-二氨基丙烷和1,8-二氨基辛烷不是前列腺酶组分的受体，但1,5-二氨基戊烷（尸胺）具有活性，且1,9-二氨基壬烷和1,12-二氨基十二烷也能导致[甲基-¹⁴C]甲硫基腺苷的生成。
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