Zelenin A V, Poletaev A I, Stepanova N G, Barsky V E, Kolesnikov V A, Nikitin S M, Zhuze A L, Gnutchev N V
Cytometry. 1984 Jul;5(4):348-54. doi: 10.1002/cyto.990050410.
A technique for DNA amount determination by flow cytometry based on the use of 7-amino-actinomycin D (7-amino-AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10(-5)M solution of 7-amino-AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%. The absorption maximum of the complex of 7-amino-AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.
