In vitro inactivation of the neurotoxic action of beta-bungarotoxin by the marine natural product, manoalide.

The irreversible neurotoxic action of beta-bungarotoxin (beta-BuTx) can be prevented by preincubation of the toxin with manoalide, a non-steroidal anti-inflammatory agent. Manoalide was also found to inactivate purified phospholipase A2 and thus prevent hydrolysis of phosphatidylcholine. PLA2 is a component found in several neurotoxic venoms and is also a rate limiting enzyme important in phospholipid metabolism and prostaglandin synthesis in man.