Bedford P A, Knight S C
Division of Immunological Medicine, Clinical Research Centre, Harrow, UK.
Clin Exp Immunol. 1989 Mar;75(3):481-6.
The effect of retinoid administration on the antigen presenting function of mouse dendritic cells (DC) was assessed. Culturing spleen cells with retinoic acid (10(-10)-10(-4) M) had no effect on the numbers of DC separated from these cultures. However, DC isolated from retinoic-acid-treated cultures were less stimulatory than DC from untreated cultures when added to allogeneic lymphocytes in a mixed leucocyte culture. Allogeneic stimulation by DC was also inhibited by pulsing separated DC with retinoic acid (10(-6)-10(-4) M) for 2 h. Pulsing DC with lower doses of retinoic acid (10(-14)-10(-20) M) enhanced this response. DC isolated from animals maintained on VAA-enriched diets had a reduced capacity to stimulate allogeneic lymphocytes. The response of unseparated lymph node cells pulsed with retinoic acid to untreated allogeneic DC was inhibited by 10(-10)-10(-4) M retinoic acid but enhanced by lower doses (10(-14) M). However, the inhibitory effect of retinoids on the function of responding lymphoid populations was abolished on removal of DC from responding cells. The results indicate that immunomodulation by retinoids could occur via an effect on the efficiency of antigen presentation by DC.
评估了类视黄醇给药对小鼠树突状细胞（DC）抗原呈递功能的影响。用视黄酸（10^(-10)-10^(-4)M）培养脾细胞对从这些培养物中分离出的DC数量没有影响。然而，当在混合淋巴细胞培养中添加到同种异体淋巴细胞时，从视黄酸处理的培养物中分离出的DC比未处理培养物中的DC刺激作用更小。用视黄酸（10^(-6)-10^(-4)M）脉冲分离的DC 2小时也会抑制DC的同种异体刺激。用较低剂量的视黄酸（10^(-14)-10^(-20)M）脉冲DC会增强这种反应。从富含VAA的饮食喂养的动物中分离出的DC刺激同种异体淋巴细胞的能力降低。用视黄酸脉冲的未分离淋巴结细胞对未处理的同种异体DC的反应被10^(-10)-10^(-4)M视黄酸抑制，但被较低剂量（10^(-14)M）增强。然而，当从反应细胞中去除DC时，类视黄醇对反应性淋巴细胞群体功能的抑制作用就会消失。结果表明，类视黄醇的免疫调节作用可能是通过对DC抗原呈递效率的影响而发生的。
