Schill W B, Trotnow S
Hautarzt. 1984 Jun;35(6):313-5.
A case of a man with a malignant testicular tumor is reported; during the time between orchiectomy and retroperitoneal lymphadenectomy his semen was preserved and stored in liquid nitrogen (-196 degrees C). Due to postoperative ejaculation disturbance resulting in infertility of the husband, artificial insemination with cryopreserved semen was performed over a period of 9 months without leading to conception. During a laparoscopy and perturbation, which were combined with an attempt for in vitro fertilization (IVF), three oocytes were collected and fertilized in vitro by cryopreserved spermatozoa of the husband. Two days later two embryos were transferred, unfortunately resulting in a bilateral tubal pregnancy. The case report demonstrates that less than 10,000 progressively motile spermatozoa are sufficient to perform successful IVF and embryo transfer.
报道了一例患有恶性睾丸肿瘤的男性病例；在睾丸切除术和腹膜后淋巴结清扫术期间，他的精液被保存并储存在液氮（-196摄氏度）中。由于术后射精障碍导致丈夫不育，在9个月的时间里进行了冷冻精液人工授精，但未成功受孕。在腹腔镜检查和干扰的同时尝试体外受精（IVF），收集了三个卵母细胞，并通过丈夫的冷冻精子进行体外受精。两天后移植了两个胚胎，不幸的是导致了双侧输卵管妊娠。该病例报告表明，少于10000条进行性活动精子足以成功进行IVF和胚胎移植。
