Rowland G F, Cohen J, Steptoe P C, Hewitt J
Urology. 1985 Jul;26(1):33-6. doi: 10.1016/0090-4295(85)90250-x.
Semen for cryopreservation was collected in a man with a testicular teratoma after unilateral orchidectomy but before chemotherapy which rendered him azoospermic. After two years artificial insemination using this semen in his wife failed repeatedly. The semen quality on thawing was extremely poor in terms of sperm motility. A pre-freeze motility of 90 per cent was reduced to 2 per cent, and the movement was graded as sluggish. Using the techniques of semen and oocyte preparation and in vitro fertilization, a number of cleaving embryos was produced. A pregnancy was established after four of these embryos were replaced in the wife. The pregnancy aborted spontaneously, but a subsequent course of treatment resulted in an on-going twin pregnancy. The potential of in vitro fertilization for overcoming the poor quality of semen after storage by cryopreservation from men with testicular neoplasms is discussed.
用于冷冻保存的精液采集自一名患有睾丸畸胎瘤的男性，采集时间为单侧睾丸切除术后但化疗前，化疗导致他无精子症。两年后，使用该精液对其妻子进行人工授精多次失败。解冻后的精液质量在精子活力方面极差。冷冻前90%的活力降至2%，运动被评为迟缓。使用精液和卵母细胞制备技术以及体外受精技术，产生了一些分裂胚胎。将其中4个胚胎移植到妻子体内后成功怀孕。此次怀孕自然流产，但随后的治疗过程导致了持续的双胎妊娠。本文讨论了体外受精对于克服睾丸肿瘤男性冷冻保存后精液质量差的潜力。
