Fleitman J, Neu D, Visor G
J Assoc Off Anal Chem. 1986 Jan-Feb;69(1):20-4.
A reverse phase liquid chromatographic (LC) procedure is described for quantitating oxfendazole (2-(methoxycarbonylamino)-5-phenylsulfinylbenzimidazole] in swine premix. Sample preparation consists of extracting oxfendazole with an acetone-methanol mixture. An aliquot of the extract is then centrifuged to separate undissolved premix excipients. Internal standard is added to the supernate and the sample is further diluted with water-acetonitrile-phosphoric acid (80 + 20 + 1). Oxfendazole is quantitatively determined using a Partisil-5-ODS-3 column with acetonitrile-0.01 M phosphate buffer (pH 6.0) as the mobile phase. The method is stability specific and yields a mean recovery of 101.1 +/- 0.4% for the 1.35% premix formulation. The dependence of chromatographic performance characteristics on mobile phase organic content, pH, and buffer concentration is also reported.
本文描述了一种反相液相色谱（LC）方法，用于定量猪预混料中的奥芬达唑（2-（甲氧基羰基氨基）-5-苯基亚磺酰基苯并咪唑）。样品制备包括用丙酮 - 甲醇混合物萃取奥芬达唑。然后将提取物的一份离心，以分离未溶解的预混料赋形剂。将内标加入上清液中，并用 水 - 乙腈 - 磷酸（80 + 20 + 1）进一步稀释样品。使用Partisil - 5 - ODS - 3柱，以乙腈 - 0.01 M磷酸盐缓冲液（pH 6.0）作为流动相，对奥芬达唑进行定量测定。该方法具有稳定性特异性，对于1.35%的预混料制剂，平均回收率为101.1±0.4%。还报道了色谱性能特征对流动相有机含量、pH和缓冲液浓度的依赖性。
