通过改进的蛋白质-染料结合测定法定量亚微克量的蛋白质。
Quantitation of submicrogram quantities of protein by an improved protein-dye binding assay.
作者信息
Bearden J C
出版信息
Biochim Biophys Acta. 1978 Apr 26;533(2):525-9. doi: 10.1016/0005-2795(78)90398-7.
PMID:647023
Abstract
A quantitative assay method for protein is described which is based on the color change occurring in Coomassie Brilliant Blue G-250 when it binds to protein. This modification of two similar procedures further increases the sensitivity, simplicity, and stability of the protein-dye binding assay over those of other commonly used assays for protein.
摘要
本文描述了一种蛋白质定量测定方法，该方法基于考马斯亮蓝G - 250与蛋白质结合时发生的颜色变化。对两个类似程序的这种改进进一步提高了蛋白质 - 染料结合测定法相对于其他常用蛋白质测定法的灵敏度、简便性和稳定性。
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