Sydow G
Acta Biol Med Ger. 1979;38(8):1141-4.
The assay is based on the property of proteins to form a complex with bromophenol blue. In alkaline solution this complex shows an intensive blue colour which is well measurable at 598 nm. The method is especially useful for small volumes (5-50 microliter) with a content of 5-30 micrograms of protein. By modification the test can be also used for the measurement of higher amounts of protein.
该测定基于蛋白质与溴酚蓝形成复合物的特性。在碱性溶液中,这种复合物呈现出强烈的蓝色,在598纳米处可以很好地测量。该方法对于含有5至30微克蛋白质的小体积样本(5至50微升)特别有用。通过改进,该测试也可用于测量更高含量的蛋白质。