Pogadaev V I, Timin E N, Khodorov B I
Biofizika. 1978 Mar-Apr;23(2):290-5.
The experiments were performed on the depolarized smooth muscle of taenia coli with the use of double sucrose-gap arrangement. Muscle contractions were induced by 1) application of hystamin or bradikynin; 2) rectangular long-lasting (10--20 s) pulses of hyperpolarizing current--the strong contraction appeared in response to the switching off the current (off-response). Both the on- and off-responses to the hyperpolarizing current recorded before, during and after hystamine (or bradikynin)-induced contration were, as a rule, very similar. Treatment of smooth muscle with local anaesthetics (procaine, trimecaine, QX-572) removed hystamine- and bradikynin-induced contrations and only decreased off-responses. The analysis of the data obtained suggested the existence of the independent electrically and chemically excitable systems (channels?) OF Ca2+ ion transport in the membrane of smooth muscle cells.
实验采用双蔗糖间隙装置在去极化的结肠带平滑肌上进行。肌肉收缩由以下方式诱导：1）应用组胺或缓激肽；2）施加持续时间为10 - 20秒的矩形超极化电流脉冲——在电流关闭时出现强烈收缩（关闭反应）。通常，在组胺（或缓激肽）诱导收缩之前、期间和之后记录的对超极化电流的开启和关闭反应非常相似。用局部麻醉剂（普鲁卡因、三甲卡因、QX - 572）处理平滑肌可消除组胺和缓激肽诱导的收缩，且仅降低关闭反应。对所得数据的分析表明，平滑肌细胞膜中存在独立的电和化学可兴奋系统（通道？）用于Ca2 +离子转运。
