Colditz I G, Movat H Z
J Immunol. 1984 Oct;133(4):2169-73.
The influx of neutrophils into cutaneous lesions induced with the chemotaxins formylmethionyl-leucyl-phenylalanine (FMLP), platelet-activating factor (PAF), and alpha-casein, and the chemotaxinigens endotoxin and zymosan peaked at 2 to 4 hr and then rapidly declined. In contrast, concanavalin A (Con A) induced a biphasic influx of neutrophils with an initial peak at 2 hr and a subsequent, prolonged peak between 6 and 10 hr. When the kinetics of the neutrophil influx into lesions induced with FMLP at 10(-5) M, 10(-6.5) M and 10(-8) M were examined, it was found that a comparable proportion of the total cell influx entered lesions induced with each concentration of FMLP at each time point. This result indicates that the kinetics of the neutrophil influx into an inflammatory lesion is not dependent on the concentration of chemotaxin within the lesion. The inflammatory potency of FMLP, PAF, alpha-casein, Con A, and endotoxin were compared with leukotriene B4 (LTB4) and zymosan-activated plasma (ZAP) as a source of C5 fragments. Equipotent concentrations were 10(-4) M alpha-casein, 10(-5) M PAF, 10(-5.5) M LTB4 and Con A, 10(-6) M FMLP, 10(-7) M ZAP, and 10(-11) M endotoxin.
用趋化因子甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）、血小板活化因子（PAF）和α-酪蛋白以及趋化原内毒素和酵母聚糖诱导皮肤损伤时，中性粒细胞的流入在2至4小时达到峰值，然后迅速下降。相比之下，伴刀豆球蛋白A（Con A）诱导中性粒细胞出现双相流入，最初在2小时达到峰值，随后在6至10小时出现延长的峰值。当检测10⁻⁵M、10⁻⁶.⁵M和10⁻⁸M的FMLP诱导损伤时中性粒细胞流入的动力学时，发现在每个时间点，每种浓度的FMLP诱导损伤时，进入损伤部位的总细胞流入比例相当。该结果表明，中性粒细胞流入炎症损伤部位的动力学不依赖于损伤部位趋化因子的浓度。将FMLP、PAF、α-酪蛋白、Con A和内毒素的炎症效力与白三烯B4（LTB4）和作为C5片段来源的酵母聚糖激活血浆（ZAP）进行比较。等效浓度分别为10⁻⁴Mα-酪蛋白、10⁻⁵M PAF、10⁻⁵.⁵M LTB4和Con A、10⁻⁶M FMLP、10⁻⁷M ZAP和10⁻¹¹M内毒素。
