用针对合成肽的抗体中和诺氏疟原虫子孢子的感染性
Neutralization of the infectivity of sporozoites of Plasmodium knowlesi by antibodies to a synthetic peptide.
作者信息
Gysin J, Barnwell J, Schlesinger D H, Nussenzweig V, Nussenzweig R S
出版信息
J Exp Med. 1984 Sep 1;160(3):935-40. doi: 10.1084/jem.160.3.935.
Abstract
Antibodies against a synthetic peptide representing the repetitive epitope of the circumsporozoite protein (CS) of Plasmodium knowlesi have properties similar to those of antibodies against the native protein. Either antibody reacts with the synthetic peptide, cross-links the CS protein on the membrane of the parasite giving the CSP reaction, and neutralizes the infectivity of sporozoites. The synthetic peptide and sporozoite extracts were equally effective when used in an immunoradiometric assay as antigens to detect antibodies to CS proteins. It is likely that the corresponding synthetic repeats from the human malaria parasites could be used to measure levels of anti-sporozoite antibodies in endemic areas, or to evaluate the humoral response to anti-sporozoite vaccines. The authors are grateful to Dr. Robert Gwadz, NIH, for supplying Anopheles mosquitoes and P. knowlesi sporozoites used in this study.
摘要
针对代表诺氏疟原虫环子孢子蛋白（CS）重复表位的合成肽的抗体，具有与针对天然蛋白的抗体相似的特性。两种抗体均与合成肽发生反应，使寄生虫膜上的CS蛋白交联，产生CSP反应，并中和子孢子的感染性。当用于免疫放射分析作为检测CS蛋白抗体的抗原时，合成肽和子孢子提取物同样有效。人类疟原虫相应的合成重复序列很可能可用于测量流行地区抗子孢子抗体的水平，或评估对抗子孢子疫苗的体液反应。作者感谢美国国立卫生研究院的罗伯特·格瓦兹博士提供本研究中使用的按蚊和诺氏疟原虫子孢子。
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