Grillot D, Valmori D, Lambert P H, Corradin G, Del Giudice G
World Health Organization-Immunology Research and Training Center, Department of Pathology, University of Geneva, Switzerland.
Infect Immun. 1993 Jul;61(7):3064-7. doi: 10.1128/iai.61.7.3064-3067.1993.
Multiple-antigen peptide (MAP) constructs containing different T- and B-cell epitopes were assessed for their ability to be specifically recognized by murine and human T-cell clones. The different synthetic MAP constructs consisted of a malaria T-cell epitope or of a human universal tetanus toxin helper T-cell epitope collinearly synthesized with B-cell epitopes from the circumsporozoite proteins of different malaria parasites. All constructs were able to stimulate specifically T-cell clones. Interestingly, T-cell epitopes assembled as MAP constructs did not require processing for the specific stimulation of murine and human T-cell clones, as shown by retention of their stimulatory effect in the presence of glutaraldehyde-fixed antigen-presenting cells. However, processing was required for most of the synthetic constructs containing both T- and B-cell epitopes. Thus, the requirement for processing of these constructs seems to be dictated by the nature of the B-cell epitope present.
对含有不同T细胞和B细胞表位的多抗原肽（MAP）构建体进行了评估，以确定它们被小鼠和人类T细胞克隆特异性识别的能力。不同的合成MAP构建体由疟疾T细胞表位或与来自不同疟原虫环子孢子蛋白的B细胞表位共线合成的人类通用破伤风毒素辅助性T细胞表位组成。所有构建体均能够特异性刺激T细胞克隆。有趣的是，组装成MAP构建体的T细胞表位在戊二醛固定的抗原呈递细胞存在的情况下仍保留其刺激作用，这表明其对小鼠和人类T细胞克隆的特异性刺激不需要加工处理。然而，大多数同时含有T细胞和B细胞表位的合成构建体需要加工处理。因此，这些构建体对加工处理的需求似乎取决于所存在的B细胞表位的性质。
