Kley H K, Rick W
J Clin Chem Clin Biochem. 1984 May;22(5):371-8.
In the analysis of steroid hormones careful attention is usually paid to blood collection and plasma storage. However, the appropriate care of samples cannot always be assured in routine work with steroids. Therefore, the stability of cortisol, aldosterone, 17-hydroxyprogesterone, testosterone, androstenedione, dehydroepiandrosteronesulphate, oestrone, oestradiol, sex hormone binding globulin (SHBG) and, the binding of testosterone and cortisol to plasma proteins in blood and plasma were studied before and after various handling procedures. Ten cycles of alternate freezing and thawing of plasma did not significantly affect the levels of the steroids or their plasma binding. The greatest differences, compared with controls, were seen for aldosterone (-6.2%) and oestradiol (-5.3%). Plasma storage at -28 degrees C was hardly superior to a 4 days storage at 4 degrees C (refrigerator) or 22 degrees C (room temperature). Although androstenedione (-10.9%), oestrone (-10.2%) and oestradiol (-12.2%) levels decreased by more than 10%, the means of all analyses were still in the 2 SD range. Even SHBG and the steroid binding were only slightly affected by temperature. When whole blood was stored at 4 degrees C or 22 degrees C, the resulting values differed from those obtained with plasma, but the differences were usually less than 10%. Although the levels were within the 2 SD range, whole blood showed a decrease of 12.3% for aldosterone and 14.5% for androstenedione. In contrast, plasma binding of testosterone (25.9%) and cortisol (15.1%) were substantially affected by storage at 22 degrees C in whole blood. It is concluded that repeated freezing and thawing of plasma, or storage at various temperatures have only a small effect on the measured levels of steroids and their plasma binding. Although it is not advisable, even whole blood may be used for the analysis of steroid concentrations.
在甾体激素分析中，通常会特别关注血液采集和血浆储存。然而，在甾体激素的日常检测工作中，并非总能确保对样本进行恰当的处理。因此，研究了皮质醇、醛固酮、17 - 羟孕酮、睾酮、雄烯二酮、硫酸脱氢表雄酮、雌酮、雌二醇、性激素结合球蛋白（SHBG）以及睾酮和皮质醇与血液及血浆中血浆蛋白的结合在各种处理程序前后的稳定性。血浆进行十次交替冻融循环对甾体激素水平及其血浆结合情况无显著影响。与对照组相比，醛固酮（-6.2%）和雌二醇（-5.3%）的差异最为明显。血浆在-28℃储存并不比在4℃（冰箱）或22℃（室温）储存4天更具优势。尽管雄烯二酮（-10.9%）、雌酮（-10.2%）和雌二醇（-12.2%）水平下降超过10%，但所有分析的均值仍在2个标准差范围内。即使是SHBG和甾体激素结合情况也仅受温度轻微影响。当全血在4℃或22℃储存时，所得值与血浆所得值不同，但差异通常小于10%。尽管这些水平在2个标准差范围内，但全血中醛固酮水平下降了12.3%，雄烯二酮水平下降了14.5%。相比之下，全血在22℃储存时，睾酮（25.9%）和皮质醇（15.1%）的血浆结合情况受到显著影响。结论是，血浆反复冻融或在不同温度下储存对甾体激素的测定水平及其血浆结合情况影响较小。虽然不建议，但全血甚至也可用于甾体激素浓度的分析。
