[关于5-甲基胞嘧啶及其核苷掺入高等植物DNA的不可能性]
[On the impossibility of the incorporation of 5-methylcytosine and its nucleosides into higher plant DNA].
作者信息
Sulimova G E, Vaniushin B F, Khoíka L, Fridrikh A, Bulgakov R, Cherny B
出版信息
Biokhimiia. 1978 Feb;43(2):240-5.
No radioactivity was detected in 5-methylcytosine isolated from wheat DNA after incubation of wheat seedlings with 3H-labelled 5-methylcytosine, 5-methylcytidine and 5-methyldeoxycytidine. No label from 3H-5-methylcytosine was found in DNA of seedlings. After incubation of seedlings with 3H-labelled nucleosides of 5-methylcytosine, radioactivity was discovered only in thymine of DNA. Thus 5-methylcytosine and its nucleosides can not be used in plants as direct precursors of 5-methyl cytosine residues in DNA, but nucleosides of 5-methylcytosine may be deaminated to thymidine (or deoxythymidine) and subsequently incorporated into DNA.
用³H标记的5-甲基胞嘧啶、5-甲基胞苷和5-甲基脱氧胞苷处理小麦幼苗后，从小麦DNA中分离出的5-甲基胞嘧啶未检测到放射性。在幼苗的DNA中未发现来自³H-5-甲基胞嘧啶的标记。用³H标记的5-甲基胞嘧啶核苷处理幼苗后，仅在DNA的胸腺嘧啶中发现放射性。因此，5-甲基胞嘧啶及其核苷在植物中不能作为DNA中5-甲基胞嘧啶残基的直接前体，但5-甲基胞嘧啶核苷可能脱氨生成胸腺嘧啶核苷（或脱氧胸腺嘧啶核苷），随后掺入DNA中。
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