Effect of papaverine on organic anion transport in rat kidney cortical slices.

The present studies were conducted to further investigate a role of cyclic nucleotides for organic anion transport in rat kidney cortical slices by using papaverine as a phosphodiesterase inhibitor. Papaverine was chosen for this study because this drug is an organic cation, a transport system of which is thought to be clearly separable from an organic anion transport system. Papaverine inhibited not only p-aminohippuric acid (PAH) accumulation in the slices but also urate one. Pretreatment of the slices with papaverine was effective for significant inhibition of PAH accumulation during subsequent incubation in the absence of papaverine. The present results suggest that a relationship may exist between cyclic nucleotides and organic anion transport in rat kidney cortical slices, although further work is necessary before the above evaluation can be given.