Nakashima M, Mikuriya N, Gemba M
J Pharmacobiodyn. 1984 May;7(5):342-5. doi: 10.1248/bpb1978.7.342.
The present studies were conducted to further investigate a role of cyclic nucleotides for organic anion transport in rat kidney cortical slices by using papaverine as a phosphodiesterase inhibitor. Papaverine was chosen for this study because this drug is an organic cation, a transport system of which is thought to be clearly separable from an organic anion transport system. Papaverine inhibited not only p-aminohippuric acid (PAH) accumulation in the slices but also urate one. Pretreatment of the slices with papaverine was effective for significant inhibition of PAH accumulation during subsequent incubation in the absence of papaverine. The present results suggest that a relationship may exist between cyclic nucleotides and organic anion transport in rat kidney cortical slices, although further work is necessary before the above evaluation can be given.
本研究旨在通过使用罂粟碱作为磷酸二酯酶抑制剂，进一步探讨环核苷酸在大鼠肾皮质切片中有机阴离子转运中的作用。本研究选择罂粟碱是因为该药物是一种有机阳离子，其转运系统被认为与有机阴离子转运系统明显不同。罂粟碱不仅抑制切片中对氨基马尿酸（PAH）的积累，也抑制尿酸盐的积累。在无罂粟碱的情况下随后孵育期间，用罂粟碱预处理切片可有效显著抑制PAH的积累。目前的结果表明，大鼠肾皮质切片中环核苷酸与有机阴离子转运之间可能存在关联，尽管在给出上述评估之前还需要进一步的研究。
