Isolation and cultivation of mature oligodendroglial cells.

CNS axons are ensheathed by myelin which is produced and maintained by oligodendrocytes. A disorder of this assembly results in functional disturbances, e.g., paralysis in multiple sclerosis. Methods are now available to isolate and cultivate oligodendrocytes in vitro. Thus, basic oligodendroglial properties can be now investigated: signals for oligodendroglial gene expression and their role in myelinogenesis and the interaction between oligodendrocytes and other neural cells by, e.g., the release of informational substances.