Merolli Antonio, Mao Yong, Kohn Joachim
New Jersey Center for Biomaterials, Rutgers-The State University of New Jersey, 145 Bevier Rd., Piscataway, NJ, 08854, USA.
Policlinico Gemelli, Universita' Cattolica del Sacro Cuore, largo Gemelli 8, 00168, Rome, Italy.
J Mater Sci Mater Med. 2017 Apr;28(4):57. doi: 10.1007/s10856-017-5867-x. Epub 2017 Feb 16.
Understanding of myelination/remyelination process is essential to guide tissue engineering for nerve regeneration. In vitro models currently used are limited to cell population studies and cannot easily identify individual cell contribution to the process. We established a novel model to study the contribution of human Schwann cells to the myelination process. The model avoids the presence of neurons in culture; Schwann cells respond solely to the biophysical properties of an artificial axon. The model uses a single carbon fiber suspended in culture media far from the floor of the well. The fiber provides an elongated structure of defined diameter with 360-degree of surface available for human Schwann cells to wrap around. This model enabled us to spatially and temporally track the myelination by individual Schwann cells along the fiber. We observed cell attachment, elongation and wrapping over a period of 9 days. Cells remained alive and expressed Myelin Basic Protein and Myelin Associated Glycoprotein as expected. Natural and artificial molecules, and external physical factors (e.g., p atterned electrical impulses), may be tested with this model as possible regulators of myelination.
了解髓鞘形成/再髓鞘形成过程对于指导神经再生的组织工程至关重要。目前使用的体外模型仅限于细胞群体研究，无法轻易确定单个细胞对该过程的贡献。我们建立了一种新型模型来研究人雪旺细胞对髓鞘形成过程的贡献。该模型避免了培养物中神经元的存在；雪旺细胞仅对人工轴突的生物物理特性作出反应。该模型使用一根悬浮在远离孔底部的培养基中的单根碳纤维。该纤维提供了一个具有确定直径的细长结构，其360度表面可供人雪旺细胞包裹。该模型使我们能够在空间和时间上追踪单个雪旺细胞沿纤维的髓鞘形成过程。我们观察了9天内细胞的附着、伸长和包裹情况。细胞保持存活，并如预期那样表达髓鞘碱性蛋白和髓鞘相关糖蛋白。天然和人工分子以及外部物理因素（例如， patterned electrical impulses，此处原文可能有误，推测为“patterned electrical impulses”，即“ patterned electrical impulses”可能是“patterned electrical impulses”，意为“ patterned electrical impulses”可能是“ patterned electrical impulses”，意为“有图案的电脉冲” ）可以用该模型作为髓鞘形成的可能调节因子进行测试。 （最后关于“patterned electrical impulses”不太明确准确意思，按照字面猜测翻译了，可能需要根据正确原文调整）
