[大鼠肝脏酸溶性部分核苷酸的分级分离]
[Fractionation of the nucleotides of the acid-soluble liver fraction of rats].
作者信息
Khokha A M, Fustochenko B P
出版信息
Prikl Biokhim Mikrobiol. 1984 Jul-Aug;20(4):565-70.
PMID:6473312
Abstract
A method for separation of rat liver acid-soluble nucleotides was developed including ion exchange polyethyleneimine cellulose chromatography, followed by rechromatography of the separate fractions on Dowex 1 and Aminex MS resins. It is simple, reproducible and does not require expensive reagents and devices. The sensitivity of the method in respect to orotate is 100 pM in a sample. Data on the content of liver basic 5'-ribonucleotides and their derivatives were obtained by the proposed method.
摘要
开发了一种分离大鼠肝脏酸溶性核苷酸的方法，包括离子交换聚乙烯亚胺纤维素色谱法，随后将分离出的组分在Dowex 1和Aminex MS树脂上进行再色谱分析。该方法简单、可重复，不需要昂贵的试剂和设备。该方法对乳清酸的检测灵敏度在样品中为100 pM。通过所提出的方法获得了肝脏碱性5'-核糖核苷酸及其衍生物含量的数据。
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