Yoshida K, Kondo F
Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Japan.
Microbios. 1994;77(311):111-9.
Using two different ion-exchange resins (Dowex 50W-X4 as cation and Dowex 1-X4 as anion) added directly to assay plates seeded with Bacillus subtilis or Micrococcus luteus, the size of the inhibitory zone produced by 36 antimicrobial agents around a disc or cup was characterized into various types, such as acidic, basic or amphoteric. An increase of the inhibition zone following addition of 15% Dowex 50W-X4 was evident in penicillins except for ampicillin and penicillin-G, and polyethers. Aminoglycosides, macrolides and colistin, lincomycin, and sulphonamides on assay medium treated with Dowex 1-X4 showed a similar effect on the inhibition zone. Tetracyclines, virginiamycin, oxolinic acid and furazoridone revealed no effects on the inhibition zone with either of the resins. These antibiotics could be divided into various groups on the basis of their chemical structure. This simple and rapid method may be useful for routine laboratory testing of residual antibiotics in meat.
将两种不同的离子交换树脂（Dowex 50W-X4作为阳离子树脂，Dowex 1-X4作为阴离子树脂）直接添加到接种了枯草芽孢杆菌或藤黄微球菌的测定平板中，对圆盘或杯周36种抗菌剂产生的抑菌圈大小进行分类，分为酸性、碱性或两性等不同类型。除氨苄青霉素和青霉素G以及聚醚类外，添加15% Dowex 50W-X4后，青霉素类抗生素的抑菌圈明显增大。在用Dowex 1-X4处理的测定培养基上，氨基糖苷类、大环内酯类、黏菌素、林可霉素和磺胺类药物对抑菌圈有类似影响。四环素类、维吉尼亚霉素、恶喹酸和痢特灵对两种树脂处理后的抑菌圈均无影响。这些抗生素可根据其化学结构分为不同类别。这种简单快速的方法可能有助于肉类中残留抗生素的常规实验室检测。
