Mattsby-Baltzer I, Gemski P, Alving C R
Rev Infect Dis. 1984 Jul-Aug;6(4):444-8. doi: 10.1093/clinids/6.4.444.
In a previous study, lipid A from Shigella flexneri was separated into eight distinct fractions by thin-layer chromatography (TLC). In the present investigation, lipid A from different gram-negative bacteria was analyzed by thin-layer chromatography for its heterogeneity and compared with lipid A of S. flexneri. Purified lipid A preparations from six different enterobacterial species were heterogeneous, but based on Rf, comparable bands were found among all preparations. Changing the culture conditions by using two other culture media resulted in very minor changes in the TLC appearance. Different serotypes of Shigella grown under the same conditions also showed very minor differences. Two bands (5 and 8) in each preparation of lipid A comigrated with lysophosphatidylethanolamine and phosphatidylethanolamine, respectively, and both bands were stained with ninhydrin and molybdenum blue. Therefore, phosphatidylethanolamine, and probably lysophosphatidylethanolamine, are common constitutents of unfractioned lipid A preparations, and both phospholipids can occur even in highly purified unfractionated lipid A.
在之前的一项研究中，福氏志贺菌的脂多糖通过薄层色谱法（TLC）被分离成八个不同的组分。在本研究中，对来自不同革兰氏阴性菌的脂多糖进行了薄层色谱分析，以研究其异质性，并与福氏志贺菌的脂多糖进行比较。从六种不同肠道细菌物种中纯化得到的脂多糖制剂具有异质性，但基于比移值（Rf），在所有制剂中发现了可比的条带。使用另外两种培养基改变培养条件，导致薄层色谱外观的变化非常小。在相同条件下生长的不同血清型志贺菌也显示出非常小的差异。脂多糖各制剂中的两条带（5号和8号）分别与溶血磷脂酰乙醇胺和磷脂酰乙醇胺共迁移，并且这两条带都能被茚三酮和钼酸蓝染色。因此，磷脂酰乙醇胺，可能还有溶血磷脂酰乙醇胺，是未分级脂多糖制剂的常见成分，并且即使在高度纯化的未分级脂多糖中这两种磷脂也可能存在。
