Schmid P C, Pfeiffer D R, Schmid H H
J Lipid Res. 1981 Jul;22(5):882-6.
A microanalytical procedure for the determination of trace amounts of lysophosphatidylethanolamine and other amine-containing lipids is described. The technique involves reaction of lipid extracts with fluorescamine to give fluorescent derivatives of lipids containing a free amino group, separation of the products by thin-layer chromatography, and quantification by fluorescence spectroscopy after elution from the adsorbent. Amounts of 100 pmol are easily determined, even in the presence of large amounts of other phospholipids, and 30-40 samples can be analyzed per day. Use of the procedure is illustrated with rat liver mitochondria undergoing a phospholipase A2-dependent increase in inner membrane permeability due to treatment with Ca2+ plus N-ethylmaleimide.
本文描述了一种用于测定痕量溶血磷脂酰乙醇胺和其他含胺脂质的微量分析方法。该技术包括脂质提取物与荧光胺反应，生成含游离氨基脂质的荧光衍生物，通过薄层色谱分离产物，并在从吸附剂上洗脱后用荧光光谱法定量。即使存在大量其他磷脂，也能轻松测定100皮摩尔的量，并且每天可分析30 - 40个样品。以经Ca2+加N - 乙基马来酰亚胺处理后，线粒体内膜通透性因磷脂酶A2依赖性增加的大鼠肝线粒体为例，说明了该方法的应用。
