从组织脂质提取物中对磷脂和溶血磷脂进行一维薄层色谱分离。

One-dimensional thin-layer chromatographic separation of phospholipids and lysophospholipids from tissue lipid extracts.

作者信息

Wang W Q, Gustafson A

机构信息

Department of Internal Medicine, University Hospital, Lund, Sweden.

出版信息

J Chromatogr. 1992 Oct 2;581(1):139-42. doi: 10.1016/0378-4347(92)80457-2.

DOI:10.1016/0378-4347(92)80457-2

PMID:1429997

Abstract

A modified one-dimensional thin-layer chromatographic procedure is presented for the separation from tissues of five phospholipids (phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine and sphingomyelin) and three lysophospholipids (lysophosphatidylserine, lysophosphatidylethanolamine and lysophosphatidylcholine). This is achieved by simple involvement of 0.4% ammonium sulphate in silica gel H and of acetone in a developing solvent as chloroform-methanol-acetic acid-acetone-water (40:25:7:4:2, v/v). The procedure is simple and the separation is reproducible. The weakness of this method is the partial degradation of phosphatidylethanolamine to lysophosphatidylethanolamine, but a method to prevent this degradation is also presented.

摘要

本文介绍了一种改进的一维薄层色谱法，用于从组织中分离五种磷脂（磷脂酰丝氨酸、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰胆碱和鞘磷脂）和三种溶血磷脂（溶血磷脂酰丝氨酸、溶血磷脂酰乙醇胺和溶血磷脂酰胆碱）。通过在硅胶H中简单加入0.4%硫酸铵，并在展开溶剂氯仿 - 甲醇 - 乙酸 - 丙酮 - 水（40:25:7:4:2，v/v）中加入丙酮来实现。该方法简单且分离结果具有可重复性。此方法的缺点是磷脂酰乙醇胺会部分降解为溶血磷脂酰乙醇胺，但本文也提出了一种防止这种降解的方法。