大鼠动脉壁颗粒部分释放的胆固醇酯酶对人工脂质混合物中胆固醇酯的水解作用。

Hydrolysis of cholesterol ester in artificial lipid mixtures by cholesterol esterase released from particulate fractions of rat arterial wall.

作者信息

Shirai K, Shinomiya M, Matsuoka N, Saito Y, Yoshida S

出版信息

Tohoku J Exp Med. 1984 Jun;143(2):171-6. doi: 10.1620/tjem.143.171.

DOI:10.1620/tjem.143.171

Abstract

Acid and neutral cholesterol esterase activities in rat arterial wall were released from the lysosomal fraction and microsomal fraction respectively into the 105,000 X g supernatant fraction by treatment with Triton X-100, heparin and dextran sulfate. The percentage releases of acid cholesterol esterase by Triton X-100 (0.1%), heparin (50 micrograms/ml) and dextran sulfate (1 mg/ml) were 21%, 18% and 4%, respectively, while those of neutral cholesterol esterase were 66%, 56% and 39%, respectively. The cholesterol esterase released by dextran sulfate, especially that from the microsomal fraction, hydrolyzed cholesterol ester in artificial lipid mixtures with similar lipid compositions to those of the deposits in fatty streaks and fibrous plaques of atheromatous lesions.

摘要

通过用 Triton X - 100、肝素和硫酸葡聚糖处理，大鼠动脉壁中的酸性和中性胆固醇酯酶活性分别从溶酶体部分和微粒体部分释放到105,000×g 上清液部分。Triton X - 100（0.1%）、肝素（50微克/毫升）和硫酸葡聚糖（1毫克/毫升）对酸性胆固醇酯酶的释放百分比分别为21%、18%和4%，而对中性胆固醇酯酶的释放百分比分别为66%、56%和39%。硫酸葡聚糖释放的胆固醇酯酶，尤其是来自微粒体部分的酶，能水解人工脂质混合物中的胆固醇酯，该脂质混合物的脂质组成与动脉粥样硬化病变的脂肪条纹和纤维斑块中的沉积物相似。