Subbiah M T
Steroids. 1979 Mar;33(3):305-15. doi: 10.1016/0039-128x(79)90007-2.
This study for the first time has simultaneously assayed three cholesteryl ester hydrolase activities located in the various subcellular fractions (lysosomal, microsomal, and soluble) of the aorta and their significance in aortic cholesteryl ester accumulation during genetic and cholesterol-fed atherosclerosis is assessed. When the enzyme activities in the aorta of age-matched atherosclerosis-susceptible White Carneau and atherosclerosis-resistant Show Racer pigeons were compared, a decrease in microsomal cholesteryl ester hydrolase activity was found during the period of cholesteryl ester accumulation. However, under cholesterol-fed conditions (which further increase cholesteryl ester accumulation), an increase in lysosomal cholesteryl ester hydrolase activity and a decrease in soluble cholesteryl ester hydrolase activity was found. These studies have documented differences in response in specific cholesteryl ester hydrosases of the aorta to genetic and cholesterol-fed atherogenesis and warrant further studies to investigate the effect of hormonal and dietary factors on the activities of these enzymes.
本研究首次同时测定了位于主动脉不同亚细胞组分（溶酶体、微粒体和可溶性组分）中的三种胆固醇酯水解酶活性，并评估了它们在遗传性和高胆固醇喂养诱导的动脉粥样硬化过程中对主动脉胆固醇酯积累的意义。当比较年龄匹配的动脉粥样硬化易感白卡诺鸽和抗动脉粥样硬化赛鸽主动脉中的酶活性时，发现在胆固醇酯积累期间微粒体胆固醇酯水解酶活性降低。然而，在高胆固醇喂养条件下（这会进一步增加胆固醇酯积累），发现溶酶体胆固醇酯水解酶活性增加，可溶性胆固醇酯水解酶活性降低。这些研究记录了主动脉中特定胆固醇酯水解酶对遗传性和高胆固醇喂养诱导的动脉粥样硬化反应的差异，值得进一步研究以探讨激素和饮食因素对这些酶活性的影响。
