Miyahara T, Sugiyama S, Kaji T, Yamashita R, Oh-e Y, Kurano T, Kozuka H
Toxicol Appl Pharmacol. 1984 Sep 30;75(3):479-84. doi: 10.1016/0041-008x(84)90184-4.
To investigate the interaction between Cd and Cu in relation to the content of collagen in bone, femurs obtained from 9-day-old chick embryos were cultivated with a combination of Cd and Cu concentrations of 8.9 microM and below for 4 days. When 2.23 or 4.45 microM Cd was added to the medium containing 4.45 or 8.90 microM Cu, the presence of both Cd and Cu caused a remarkable decrease in collagen synthesis compared with a decrease in collagen synthesis caused by either Cd or Cu alone. The results show that Cd and Cu caused an interactive inhibition of collagen synthesis, which was not due to an increase in collagen degradation by Cd and Cu. At concentrations showing inhibition, Cd caused an increase in Cu content and Cu caused an increase in Cd content. The increase in Cd content was mainly caused by the accumulation of Cd in bone mineral. The increase in Cu content was due to binding to metallothionein-like protein in the cytosol induced by Cd. The relationship between inhibition and the increase in both Cd and Cu was not confirmed after division of the bone into diaphysis and epiphysis. The present study showed that Cu aggravated the bone matrix damage caused by Cd.
为研究镉（Cd）与铜（Cu）的相互作用对骨中胶原蛋白含量的影响，将9日龄鸡胚的股骨在Cd和Cu浓度均为8.9微摩尔及以下的组合条件下培养4天。当向含有4.45或8.90微摩尔Cu的培养基中添加2.23或4.45微摩尔Cd时，与单独的Cd或Cu所导致的胶原蛋白合成减少相比，Cd和Cu同时存在会使胶原蛋白合成显著减少。结果表明，Cd和Cu对胶原蛋白合成产生了交互抑制作用，这并非是由于Cd和Cu导致胶原蛋白降解增加所致。在表现出抑制作用的浓度下，Cd会导致Cu含量增加，而Cu会导致Cd含量增加。Cd含量的增加主要是由于Cd在骨矿物质中的积累。Cu含量的增加是由于Cd诱导细胞溶质中与金属硫蛋白样蛋白结合所致。将骨分为骨干和骨骺后，未证实抑制作用与Cd和Cu含量增加之间的关系。本研究表明，Cu加剧了Cd对骨基质的损伤。
