Abe P M, Holland J W, Stauffer L R
Am J Vet Res. 1978 Jan;39(1):115-8.
Immunization of mice against Fusobacterium necrophorum infection was attempted by using 3 vaccination procedures: (1) intraperitoneal (IP) injection of F necrophorum cells in saline solution, (2) IP injection of cells with added aluminum hydroxide adjuvant, and (3) feeding of a powdered mouse diet containing lyophilized cells. One or 2 weekly IP injections of the bacteria cells (in saline solution) for 3, 6, or 12 weeks resulted in protection of 48.7% to 64.5% of the mice against challenge exposure. Of the 2 control groups (given saline solution only), 100% and 97.4% became infected. Weekly IP injections of bacterial cells in an aluminum hydroxide adjuvant for 3, 6, or 12 weeks resulted in protectivity of 54.1% to 77.5%. Of the control mice (given adjuvant only), 97.5% became infected. Bacterial cells fed to mice at a dose level of 1.5 mg (dry weight)/g of powdered diet for 30 days (4 or 5g of diet each day) resulted in only a delay in the mean time of death as compared with the rapid death of the control mice. The feeding dose of 0.15 mg of cells/g of diet did not delay the mean time of death.
（1）腹腔内（IP）注射盐溶液中的坏死梭杆菌细胞，（2）腹腔内注射添加氢氧化铝佐剂的细胞，以及（3）喂食含冻干细胞的粉状小鼠饲料。每周进行1次或2次腹腔内注射细菌细胞（在盐溶液中），持续3、6或12周，结果显示48.7%至64.5%的小鼠受到保护，免受激发暴露感染。在2个对照组（仅给予盐溶液）中，100%和97.4%的小鼠受到感染。每周腹腔内注射含氢氧化铝佐剂的细菌细胞，持续3、6或12周，保护率为54.1%至77.5%。在对照小鼠（仅给予佐剂）中，97.5%的小鼠受到感染。以1.5毫克（干重）/克粉状饲料的剂量水平给小鼠喂食细菌细胞30天（每天4或5克饲料），与对照小鼠的快速死亡相比，仅导致平均死亡时间延迟。0.15毫克细胞/克饲料的喂食剂量并未延迟平均死亡时间。
