Lebedev O E, Sergeenko N G, Barskiĭ I Ia, Papaian G V
Tsitologiia. 1984 May;26(5):588-93.
As has been previously reported an increased intensity of light-induced green fluorescence is observed for some tumor cells. The present paper deals with the cause of this phenomenon, employing for this hepatoma cells of line HTC acted upon with 2,4-DNP, amytal and malonate. It has been shown that the light-induced increase in green fluorescence in cells is due to the oxidation of NADH-dehydrogenase, a mitochondrial flavine-containing enzyme, occurring at the time of fluorescence induction. The increased intensity of green fluorescence of flavoproteins in tumor cells is associated with an infringement in oxidation of NAD-dependent substrates in these, and with the activation of the reverse electron transport in the oxidative chain. The exciding light activates NADH-dehydrogenase and accelerates the translocation of reduced equivalents from this enzyme, which results in its oxidation, and thus--in the observed effect of increased intensity of green fluorescence.
如先前报道，在某些肿瘤细胞中观察到光诱导的绿色荧光强度增加。本文探讨了这一现象的原因，为此采用了用2,4 -二硝基苯酚、戊巴比妥和丙二酸作用的HTC肝癌细胞系。研究表明，细胞中光诱导的绿色荧光增加是由于荧光诱导时发生的NADH脱氢酶（一种含线粒体黄素的酶）的氧化。肿瘤细胞中黄素蛋白绿色荧光强度的增加与这些细胞中NAD依赖底物氧化的损害以及氧化链中反向电子传递的激活有关。激发光激活NADH脱氢酶并加速该酶还原当量的转运，这导致其氧化，从而产生观察到的绿色荧光强度增加的效应。
