Salomatin V V, Lifshits R I
Vopr Med Khim. 1984 May-Jun;30(3):120-4.
A fraction of toxic ninhydrin-positive substances was isolated from urine of healthy persons and of the patients with acute burn toxemia using a multi-step technique, which involved ion exchange chromatography on Dowex resins, gel filtration on Sephadex G-15 and ultrafiltration on Diaflo UM-0.5. The daily excretion of the substances was about 4-fold higher in the patients as compared with healthy persons. The fraction contained basic peptides with molecular masses of 500-2,000 daltons. The total toxicity of these peptide was higher by an order of magnitude in the patients with burns as compared with healthy persons.
采用多步技术，从健康人和急性烧伤毒血症患者的尿液中分离出一部分茚三酮阳性有毒物质，该技术包括在Dowex树脂上进行离子交换色谱、在Sephadex G - 15上进行凝胶过滤以及在Diaflo UM - 0.5上进行超滤。与健康人相比，患者体内这些物质的日排泄量约高4倍。该组分含有分子量为500 - 2000道尔顿的碱性肽。与健康人相比，烧伤患者体内这些肽的总毒性高一个数量级。
