Fiedel B A, Vallve C, Izzi J M
Agents Actions. 1984 Jun;14(5-6):738-42. doi: 10.1007/BF01978918.
Rabbits made acute phase by sub-cutaneous trauma with 2% croton oil (in mineral oil) were tested by intradermal (ID) injection with platelet-granule extracts containing platelet-derived permeability factor (PDPF). Compared with controls, skin reactivity to PDPF was enhanced in acute phase animals 3-7 days post-trauma, a period of acute inflammation as reflected by the occurrence in the circulation of C-reactive protein; maximal skin responses were observed 3-4 days post-trauma. Individual skin sites reached maximum intensity 15 min-1 hour post-ID injection of PDPF and were sensitive to chlorpheniramine maleate, suggesting a major role for histamine. Intradermal injection of histamine revealed that acute phase animals yielded an initially more intense skin reaction, and were markedly less capable of recovering from the effects of histamine. These data suggest that in the acute phase, there exists a heightened and prolonged sensitivity to the action of histamine which can be exploited by pro-inflammatory agents such as PDPF.
通过皮下注射含血小板衍生通透性因子（PDPF）的血小板颗粒提取物，对用2%巴豆油（溶于矿物油）造成急性期反应的兔子进行皮内（ID）注射测试。与对照组相比，在创伤后3 - 7天的急性期动物中，皮肤对PDPF的反应性增强，这一急性期炎症阶段可通过循环中C反应蛋白的出现来反映；在创伤后3 - 4天观察到最大皮肤反应。皮内注射PDPF后，各个皮肤部位在15分钟至1小时后达到最大强度，并且对马来酸氯苯那敏敏感，提示组胺起主要作用。皮内注射组胺显示，急性期动物最初产生的皮肤反应更强烈，且从组胺作用中恢复的能力明显较弱。这些数据表明，在急性期，对组胺作用存在敏感性增强和持续时间延长的情况，促炎剂如PDPF可利用这一点。
