Takasato Y, Rapoport S I, Smith Q R
Am J Physiol. 1984 Sep;247(3 Pt 2):H484-93. doi: 10.1152/ajpheart.1984.247.3.H484.
The right cerebral hemisphere of the rat was perfused in situ by retrograde infusion of HCO3 saline or blood into the right external carotid artery. Infusion rate was adjusted to minimize the contribution of systemic blood to flow in the hemisphere. During perfusion with whole or artificial blood, regional cerebral blood flow and blood volume were comparable to respective values in the conscious rat, whereas perfusion with HCO3 saline increased regional flow three- to fourfold due to the low viscosity of the saline perfusate. Perfusion with whole blood for 300 S or with HCO3 saline for 60 S did not alter the permeability of the blood-brain barrier. Cerebrovascular permeability coefficients of eight nonelectrolytes ranged from 10(-8) to 10(-4) cm X S-1 and were directly proportional to the octanol-water partition coefficient of the solute. Thus the in situ brain perfusion technique is a sensitive new method to study cerebrovascular transfer in the rat and permits absolute control of perfusate composition.
通过将碳酸氢盐生理盐水或血液逆行注入大鼠右侧颈外动脉，对大鼠右侧大脑半球进行原位灌注。调整灌注速率，以尽量减少全身血液对半球血流的影响。在用全血或人工血液灌注期间，局部脑血流量和血容量与清醒大鼠的相应值相当，而用碳酸氢盐生理盐水灌注时，由于盐水灌注液的低粘度，局部血流量增加了三到四倍。用全血灌注300秒或用碳酸氢盐生理盐水灌注60秒不会改变血脑屏障的通透性。八种非电解质的脑血管通透系数范围为10（-8）至10（-4）厘米×秒-1，且与溶质的正辛醇-水分配系数成正比。因此，原位脑灌注技术是一种研究大鼠脑血管转运的灵敏新方法，并且可以绝对控制灌注液成分。
