Murakami H, Takanaga H, Matsuo H, Ohtani H, Sawada Y
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Am J Physiol Heart Circ Physiol. 2000 Sep;279(3):H1022-8. doi: 10.1152/ajpheart.2000.279.3.H1022.
Here we present a method for measuring the permeability coefficient-surface area product (PS) values at the blood-brain barrier in mice, using the in situ brain perfusion technique originally developed for rats by Takasato et al. (Am J Physiol Heart Circ Physiol 247: H484-H493, 1984). Retrograde infusion into the right external carotid artery increased the carotid perfusion pressure in proportion to the perfusion rate. Intravascular volume and cerebral perfusion fluid flow at a perfusion rate of 1.0 ml/min in mice were similar to those in rats. In addition, the contribution of systemic blood to total flow in the hemisphere was small (only 3. 2%). These findings indicated that this perfusion rate is suitable for mice. The PS values of more than 20 different compounds were determined in mice by using the in situ brain perfusion technique, and comparisons were made with data from rats. There was a close relationship (1:1) between the PS values in mice and rats, indicating that brain capillary permeabilities are similar in mice and rats.
在此，我们介绍一种利用Takasato等人（《美国生理学杂志：心脏和循环生理学》247: H484 - H493, 1984）最初为大鼠开发的原位脑灌注技术来测量小鼠血脑屏障通透性系数-表面积乘积（PS）值的方法。逆行注入右颈外动脉会使颈动脉灌注压与灌注速率成比例增加。小鼠在灌注速率为1.0 ml/min时的血管内容积和脑灌注液流量与大鼠相似。此外，全身血液对半球总血流量的贡献很小（仅3.2%）。这些发现表明该灌注速率适用于小鼠。通过原位脑灌注技术测定了小鼠中20多种不同化合物的PS值，并与大鼠的数据进行了比较。小鼠和大鼠的PS值之间存在密切关系（1:1），表明小鼠和大鼠的脑毛细血管通透性相似。
