Chow G, Schmidley J W
Arch Neurol. 1984 Oct;41(10):1084-5. doi: 10.1001/archneur.1984.04050210082020.
We created "bloody taps" by adding homologous blood to acellular samples of CSF and counted the RBCs and WBCs at specific time intervals. After two hours at room temperature (22 degrees C), 40% of the WBCs in the traumatic tap had lysed. At 4 degrees C, 15% of the cells lysed in the same period of time. After five hours at 22 degrees C, 53% of the WBCs had lysed, while refrigeration reduced this figure to 31%. The clinical implications of these data are clear. If a sample of CSF is not promptly refrigerated and analyzed, WBC lysis will result in a false impression of the number of WBCs that are present. This lysis of WBCs reduces the reliability of the corrective formula that uses CSF and blood RBC and WBC counts to estimate the number of WBCs in CSF that are attributable to contamination with blood.
我们通过向无细胞脑脊液样本中添加同源血液来制造“血性穿刺液”，并在特定时间间隔对红细胞和白细胞进行计数。在室温（22摄氏度）下放置两小时后，创伤性穿刺液中40%的白细胞发生了裂解。在4摄氏度时，同期有15%的细胞发生裂解。在22摄氏度下放置五小时后，53%的白细胞发生了裂解，而冷藏可使这一数字降至31%。这些数据的临床意义显而易见。如果脑脊液样本没有及时冷藏和分析，白细胞裂解将导致对实际存在的白细胞数量产生错误印象。白细胞的这种裂解降低了使用脑脊液和血液中红细胞及白细胞计数来估算脑脊液中因血液污染所致白细胞数量的校正公式的可靠性。
