脂肪酸对灌注大鼠心脏中亮氨酸和4-甲基-2-氧代戊酸脱羧作用的调节效应
Regulatory effects of fatty acids on decarboxylation of leucine and 4-methyl-2-oxopentanoate in the perfused rat heart.
作者信息
Buxton D B, Barron L L, Taylor M K, Olson M S
出版信息
Biochem J. 1984 Aug 1;221(3):593-9. doi: 10.1042/bj2210593.
The regulatory effects of fatty acids on the oxidative decarboxylation of leucine and 4-methyl-2-oxopentanoate were investigated in the isolated rat heart. Infusion of the long-chain fatty acid palmitate resulted in both an inactivation of the branched-chain 2-oxo acid dehydrogenase and an inhibition of the measured metabolic flux through this enzyme complex. Pyruvate addition also caused both an inactivation and an inhibition of the flux through the complex. On the other hand, the medium-chain fatty acid octanoate caused an activation of and a stimulation of flux through the branched-chain 2-oxo acid dehydrogenase when the perfusion conditions before octanoate addition maintained the enzyme complex in its inactive state. When the enzyme complex was activated before octanoate infusion, this fatty acid caused a significant inhibition of the flux through the branched-chain 2-oxo acid dehydrogenase reaction. Inclusion of glucose in the perfusion medium prevented the octanoate-mediated activation of the branched-chain 2-oxo acid dehydrogenase.
在离体大鼠心脏中研究了脂肪酸对亮氨酸和4-甲基-2-氧代戊酸氧化脱羧的调节作用。输注长链脂肪酸棕榈酸酯导致支链2-氧代酸脱氢酶失活，并抑制通过该酶复合物测得的代谢通量。添加丙酮酸也会导致该复合物失活并抑制通量。另一方面，当中链脂肪酸辛酸添加前的灌注条件使酶复合物处于失活状态时，辛酸会激活支链2-氧代酸脱氢酶并刺激其通量。当在输注辛酸之前激活酶复合物时，这种脂肪酸会显著抑制通过支链2-氧代酸脱氢酶反应的通量。在灌注培养基中加入葡萄糖可阻止辛酸介导的支链2-氧代酸脱氢酶的激活。
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