Norling B, Glimelius B, Wasteson A
Biochem J. 1984 Aug 1;221(3):845-53. doi: 10.1042/bj2210845.
A chondroitin sulphate proteoglycan capable of forming large aggregates with hyaluronic acid was identified in cultures of human glial and glioma cells. The glial- cell- and glioma-cell-derived products were mutually indistinguishable and had some basic properties in common with the analogous chondroitin sulphate proteoglycan of cartilage: hydrodynamic size, dependence on a minimal size of hyaluronic acid for recognition, stabilization of aggregates by link protein, and precipitability with antibodies raised against bovine cartilage chondroitin sulphate proteoglycan. However, they differed in some aspects: lower buoyant density, larger, but fewer, chondroitin sulphate side chains, presence of iduronic acid-containing repeating units, and absence (less than 1%) of keratan sulphate. Apparently the major difference between glial/glioma and cartilage chondroitin sulphate proteoglycans relates to the glycan rather than to the protein moiety of the molecule.
在人神经胶质细胞和神经胶质瘤细胞培养物中鉴定出一种能够与透明质酸形成大聚集体的硫酸软骨素蛋白聚糖。神经胶质细胞和神经胶质瘤细胞衍生的产物彼此无法区分，并且与软骨中类似的硫酸软骨素蛋白聚糖具有一些共同的基本特性：流体动力学大小、对透明质酸最小大小的识别依赖性、连接蛋白对聚集体的稳定作用以及用针对牛软骨硫酸软骨素蛋白聚糖产生的抗体的可沉淀性。然而，它们在某些方面有所不同：较低的浮力密度、更大但更少的硫酸软骨素侧链、含艾杜糖醛酸的重复单元的存在以及硫酸角质素的缺失（小于1%）。显然，神经胶质/神经胶质瘤和软骨硫酸软骨素蛋白聚糖之间的主要差异与聚糖而非分子的蛋白质部分有关。
