多胺刺激玉米（Zea mays L.）胚芽鞘蛋白质的磷酸化作用。

Polyamine-stimulated phosphorylation of proteins from corn (Zea mays L.) coleoptiles.

作者信息

Veluthambi K, Poovaiah B W

出版信息

Biochem Biophys Res Commun. 1984 Aug 16;122(3):1374-80. doi: 10.1016/0006-291x(84)91243-9.

Abstract

The effect of polyamines (spermine, spermidine and putrescine) on in vitro phosphorylation of proteins from corn coleoptiles was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Spermine promoted the phosphorylation of several membrane and soluble proteins and most of the proteins phosphorylated were different from those phosphorylated in the presence of calcium. Spermidine promoted the phosphorylation to a lesser extent and putrescine had very little stimulatory effect. Spermine-promoted phosphorylation of soluble proteins was dependent upon the presence of Mg2+ and was discernible at 100 microM spermine concentration.

摘要

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影法，研究了多胺（精胺、亚精胺和腐胺）对玉米胚芽鞘蛋白质体外磷酸化的影响。精胺促进了几种膜蛋白和可溶性蛋白的磷酸化，且大多数被磷酸化的蛋白与在钙存在下被磷酸化的蛋白不同。亚精胺促进磷酸化的程度较小，而腐胺的刺激作用很小。精胺促进的可溶性蛋白磷酸化依赖于Mg2+的存在，在精胺浓度为100 microM时可观察到这种作用。