Tsukitani M, Okamoto R, Fukuzaki H
Atherosclerosis. 1984 Aug;52(2):167-74. doi: 10.1016/0021-9150(84)90114-x.
We studied the effect of hypoxia on cholesterol accumulation in cultured rabbit aortic smooth muscle cells, which were incubated in a medium with normolipemic rabbit serum (NRS) or hyperlipemic rabbit serum (HRS). The cells were incubated in a humidified atmosphere of either 20% O2, 75% N2 and 5% CO2 (control cells) or 2% O2, 93% N2 and 5% CO2 (hypoxic cells). In a medium containing 20% NRS, the free cholesterol level of hypoxic cells was only a little higher than that of control cells, and there was no significant difference in esterified cholesterol content. On the other hand, in a medium containing 20% HRS, the free cholesterol level was slightly higher and the esterified cholesterol level was markedly higher in hypoxic cells compared with control cells. These results show that hypoxia promotes the accumulation of cholesterol, especially as ester, in smooth muscle cells cultured with hyperlipemic serum. These in vitro experiments indicate that hypoxia in the arterial wall associated with hyperlipidemia may play an important role in atherogenesis, although the precise mechanism remains unclear.
我们研究了缺氧对培养的兔主动脉平滑肌细胞中胆固醇积累的影响，这些细胞在含有正常血脂兔血清（NRS）或高脂血症兔血清（HRS）的培养基中孵育。细胞在20% O₂、75% N₂和5% CO₂的湿润气氛中孵育（对照细胞）或在2% O₂、93% N₂和5% CO₂的气氛中孵育（缺氧细胞）。在含有20% NRS的培养基中，缺氧细胞的游离胆固醇水平仅略高于对照细胞，酯化胆固醇含量无显著差异。另一方面，在含有20% HRS的培养基中，与对照细胞相比，缺氧细胞的游离胆固醇水平略高，酯化胆固醇水平明显更高。这些结果表明，缺氧促进了高脂血症血清培养的平滑肌细胞中胆固醇的积累，尤其是作为酯的形式。这些体外实验表明，与高脂血症相关的动脉壁缺氧可能在动脉粥样硬化形成中起重要作用，尽管确切机制尚不清楚。
