Zelenka P S, Vu N D
Dev Biol. 1984 Oct;105(2):325-9. doi: 10.1016/0012-1606(84)90289-6.
Differentiation of embryonic chicken lens epithelial cells to form lens fibers is associated with a marked decrease in both the rate of phosphatidylinositol degradation and the rate of cell division. In cells of the central region of the lens epithelium, the rate of cell division also declines with developmental age. The present study measures phosphatidylinositol degradation in cultured explants of the central lens epithelium of chicken embryos of different ages to determine the extent of the correlation between phosphatidylinositol degradation and cell division in this tissue. The results show that the rate of phosphatidylinositol degradation also decreases during development and is proportional to the rate of cell division throughout the period from 6 to 19 days of development. Furthermore, stimulating cell division in central explants of lens epithelia of 19-day-old chicken embryos by culturing them in the presence of fetal calf serum produces a proportional increase in the rate of phosphatidylinositol degradation. These findings indicate that cell division and phosphatidylinositol degradation are tightly coupled in this tissue, and raise the possibility that phosphatidylinositol metabolism may regulate some aspect of the cell cycle.
胚胎期鸡晶状体上皮细胞分化形成晶状体纤维，这与磷脂酰肌醇降解速率和细胞分裂速率的显著降低有关。在晶状体上皮中央区域的细胞中，细胞分裂速率也随着发育年龄而下降。本研究测量了不同年龄鸡胚晶状体上皮中央区域培养外植体中磷脂酰肌醇的降解情况，以确定该组织中磷脂酰肌醇降解与细胞分裂之间的相关程度。结果表明，磷脂酰肌醇降解速率在发育过程中也会降低，并且在6至19天的整个发育期间与细胞分裂速率成正比。此外，通过在胎牛血清存在的情况下培养19日龄鸡胚晶状体上皮中央外植体来刺激细胞分裂，会使磷脂酰肌醇降解速率成比例增加。这些发现表明，在该组织中细胞分裂和磷脂酰肌醇降解紧密相关，并增加了磷脂酰肌醇代谢可能调节细胞周期某些方面的可能性。
