Knorr M, Wunderlich K, Steuhl K P, Hoppe J
Department of General Ophthalmology, University of Tübingen, Germany.
Graefes Arch Clin Exp Ophthalmol. 1993 Jul;231(7):424-8. doi: 10.1007/BF00919653.
It has been reported previously that platelet-derived growth factor (PDGF) may play an important role in the regulation of lens growth and differentiation. To evaluate PDGF-induced effects at the cellular level, we investigated the response of cultured bovine lens epithelial cells (BLEC) to PDGF-AB, -AA, and -BB isoforms at the cellular level. Stimulation of BLEC with PDGF isoforms showed no increase in cell proliferation under the culture conditions of this study. In contrast, measurement of cytosolic free calcium concentration ([Ca2+]i), which has been shown to be an important second messenger for controlling multiple cellular processes in the lens, revealed a dose-dependent rise in [Ca2+]i upon stimulation with PDGF-AB and -BB isoforms. PDGF-AA used in similar concentrations was not effective. Our data suggest that PDGF-AB and -BB may play a role in the regulation of cellular functions in BLEC via modulation of intracellular calcium homeostasis.
先前已有报道称，血小板衍生生长因子（PDGF）可能在晶状体生长和分化的调节中发挥重要作用。为了在细胞水平评估PDGF诱导的效应，我们在细胞水平研究了培养的牛晶状体上皮细胞（BLEC）对PDGF-AB、-AA和-BB亚型的反应。在本研究的培养条件下，用PDGF亚型刺激BLEC未显示细胞增殖增加。相比之下，已证明细胞溶质游离钙浓度（[Ca2+]i）是控制晶状体中多种细胞过程的重要第二信使，测量结果显示，在用PDGF-AB和-BB亚型刺激后，[Ca2+]i呈剂量依赖性升高。使用相似浓度的PDGF-AA则无效。我们的数据表明，PDGF-AB和-BB可能通过调节细胞内钙稳态在BLEC的细胞功能调节中发挥作用。
