Piatigorsky J, Rothschild S S, Wollberg M
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1195-8. doi: 10.1073/pnas.70.4.1195.
Both fetal-calf serum and insulin cause cell elongation in explanted chick-lens epithelia from 6-day-old embryos. We show that 1 mug/ml of insulin, like serum, stimulates a doubling of cell length and an assembly of longitudinally oriented microtubules; colchicine treatment inhibits this cell elongation. In contrast to serum, insulin neither promotes further lens-cell elongation nor appreciably stimulates the synthesis of bulk proteins or of delta crystallin under the present conditions. These data indicate that the early morphological events of lens fiber differentiation can be initiated by insulin in a chemically defined, serum-free medium without significant affects upon protein synthesis.
胎牛血清和胰岛素均可使6日龄胚胎的离体鸡晶状体上皮细胞伸长。我们发现，1微克/毫升的胰岛素与血清一样，可刺激细胞长度加倍并促使纵向排列的微管组装；秋水仙碱处理可抑制这种细胞伸长。与血清不同的是，在目前条件下，胰岛素既不会促进晶状体细胞进一步伸长，也不会明显刺激大量蛋白质或δ晶状体蛋白的合成。这些数据表明，在化学成分明确的无血清培养基中，胰岛素可引发晶状体纤维分化的早期形态学变化，且对蛋白质合成无显著影响。
