Schramlová J, Blazek K, Marhoul Z, Singhvi A
Folia Parasitol (Praha). 1984;31(3):247-51.
Oncospheres of Taenia saginata cultivated in Leibovitz's medium on a cell monolayer from calf kidney developed up to 10th day of in vitro cultivation. Fine and often branched microvilli were observed on their surface. On day 5 of cultivation, the muscle system of hooks turned to a granular matter and on day 7 neither hooks nor their muscle system were found. The tegumental cytoplasm was not formed in the period of 7-60 days of cultivation. On days 7 and 10 of cultivation, a contact of oncospheres with the cells of monolayer was visible. On day 10 of cultivation, an extrusion of a portion of cytoplasm of surface cells filled with minute vesicles occurred. The bonds among cells, particularly in the centre of the oncosphere, were loosened in a 30-day-old culture and the cells were interconnected by long cytoplasmic bridges. Degenerative changes occurred on about day 40 of cultivation. The oncospheres cultivated in pure Leibovitz's medium lost the microvilli on their surface and the original tegumental cytoplasm turned to a fibrous layer already on day 5 of cultivation. Dystrophic changes in cells occurred from day 7 of cultivation and gradually increased.
在莱博维茨培养基中，于小牛肾细胞单层上培养的牛带绦虫六钩蚴，在体外培养至第10天时仍能生长。在其表面观察到细小且常呈分支状的微绒毛。培养第5天时，钩的肌肉系统变成颗粒状物质，到第7天时，既找不到钩也找不到其肌肉系统。在培养7 - 60天期间未形成皮层细胞质。在培养第7天和第10天时，可见六钩蚴与单层细胞接触。培养第10天时，充满微小囊泡的表面细胞的一部分细胞质被挤出。在30日龄培养物中，细胞间的连接，尤其是在六钩蚴中央的连接变得松弛，细胞通过长的细胞质桥相互连接。在培养约第40天时出现退行性变化。在纯莱博维茨培养基中培养的六钩蚴在培养第5天时其表面就失去了微绒毛，原来的皮层细胞质变成了纤维层。细胞的营养不良性变化从培养第7天开始出现并逐渐加重。
