Tournut R, Allan B J, White T T
Clin Chim Acta. 1978 Sep 1;88(2):345-53. doi: 10.1016/0009-8981(78)90441-2.
Substrate films of starch, RNA, and DNA were used to identify the isoenzymes of amylase, RNAase, and DNAase found in human ductal pancreatic juice subjected to isoelectric focusing. The pancreatic secretions from 15 patients were shown to contain as many as four isoenzymes of RNAase; the two major forms had isoelectric points of 7.87 and 7.52, and the two minor forms, of 7.25 and 6.90. Six DNAase bands were detected; the major bands had pI values of 4.86 and 4.79, and sometimes appeared as one band. The minor bands had pI values of 5.08, 5.00, 4.68, and 4.58. Purified bovine DNAase I, analyzed similarly, showed four bands (5.29, 5.19, 5.04 and 4.96). Nine isoenzymes of alpha-amylase were observed in the secretions from 15 patients. The major alpha-amylase isoenzyme had a pI value of 6.84 in 14 patients and of 7.04 in 1 patient. Secondary bands were seen with pI values of 6.23, 6.53 and 6.69. Additional isoenzymes were found with pI values of 7.16, 6.39, 6.00 and 5.78. The amylase isoenzyme with a pI value of 6.39 was found in 7 of the 8 patients with a normal pancreas or carcinoma of the pancreas, and in only 1 of 7 patients with acute or chronic pancreatitis.
利用淀粉、RNA和DNA底物膜来鉴定在经过等电聚焦的人胰管胰液中发现的淀粉酶、RNA酶和DNA酶的同工酶。结果显示，15名患者的胰腺分泌物中含有多达四种RNA酶同工酶；两种主要形式的等电点分别为7.87和7.52，两种次要形式的等电点分别为7.25和6.90。检测到六条DNA酶带；主要条带的等电点值为4.86和4.79，有时会显示为一条带。次要条带的等电点值为5.08、5.00、4.68和4.58。同样进行分析的纯化牛DNA酶I显示有四条带（5.29、5.19、5.04和4.96）。在15名患者的分泌物中观察到了九种α-淀粉酶同工酶。14名患者中主要的α-淀粉酶同工酶等电点值为6.84，1名患者的为7.04。还观察到等电点值为6.23、6.53和6.69的次要条带。另外还发现了等电点值为7.16、6.39、6.00和5.78的同工酶。等电点值为6.39的淀粉酶同工酶在8名胰腺正常或患有胰腺癌的患者中有7名被发现，而在7名患有急性或慢性胰腺炎的患者中只有1名被发现。
