Heino J, Kouri T, Jalkanen M, Lehtonen O P
Immunobiology. 1984 May;166(4-5):360-7. doi: 10.1016/S0171-2985(84)80014-5.
We were able to characterize about 25 antigenic proteins of cultured synovial fibroblasts with apparent molecular weights of 15-230 kd by SDS-electrophoresis and immunoblotting using guinea pig antiserum. Some of these proteins were bovine-serum-derived, adsorbed onto fibroblasts from culture medium. Both rheumatoid and normal sera contained natural antibodies reacting with synovial fibroblast as well as bovine serum antigens when studied with immunoblotting. The amounts of antibodies were quantitated with enzyme immunoassay, measuring IgG and IgA class antibodies against plasma membrane preparations of rheumatoid and normal synovial fibroblasts. No rheumatoid arthritis-related changes were detected in synovial fibroblast protein antigens or antibodies against them in rheumatoid patient specimens.
通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - 电泳）以及使用豚鼠抗血清进行免疫印迹，我们能够鉴定出培养的滑膜成纤维细胞中约25种抗原性蛋白质，其表观分子量为15 - 230千道尔顿。其中一些蛋白质来源于牛血清，是从培养基吸附到成纤维细胞上的。当用免疫印迹研究时，类风湿性关节炎患者血清和正常血清都含有与滑膜成纤维细胞以及牛血清抗原发生反应的天然抗体。通过酶免疫测定法定量抗体含量，检测针对类风湿性关节炎和正常滑膜成纤维细胞质膜制剂的IgG和IgA类抗体。在类风湿性关节炎患者标本中，未检测到滑膜成纤维细胞蛋白抗原或针对它们的抗体有与类风湿性关节炎相关的变化。
