McColl G J, Cram D S, Harrison L C
Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.
Arthritis Rheum. 1995 Oct;38(10):1418-28. doi: 10.1002/art.1780381009.
To identify synovial antigens that bind to serum antibodies from subjects with recent-onset rheumatoid arthritis (RA) (< 6 months of synovitis).
Soluble and insoluble fractions of Triton X-100 extracts of RA and normal synovial tissue, and normal spleen and placenta, were immunoblotted with sera from 27 patients with recent-onset RA, 13 autoimmune disease control subjects, and 13 blood bank control donors. Bound immunoglobulin was probed with 125I-labeled protein A.
Antibodies in the sera of 20 (74%) patients with recent-onset RA recognized at least 1 of 5 antigens in both a disease- and tissue-specific and nonspecific manner. Anti-La antibodies, usually associated with primary Sjögren's syndrome, were detected in 2 sera. Eight sera had increased reactivity to an IgG heavy and light chain dimer. There was strong binding of 8 sera with a 35-kd doublet and of 3 sera with a 55-kd species in RA and normal synovial lysates (insoluble fractions). Two sera uniquely recognized a 45-kd protein only in the RA synovial lysate (soluble fraction).
IgG antibodies in the sera of patients with recent-onset RA show positive immunoblots for 3 novel synovial antigens of 35-kd, 55-kd, and 45-kd, as well as for 2 previously characterized antigens (La and IgG). Thus, a variety of synovial antigens appear to be recognized by B cells even early in the clinical course of RA.
鉴定与近期发病的类风湿关节炎（RA）（滑膜炎病程<6个月）患者血清抗体结合的滑膜抗原。
用来自27例近期发病的RA患者、13例自身免疫性疾病对照受试者和13例血库对照献血者的血清，对RA和正常滑膜组织、正常脾脏及胎盘的Triton X-100提取物的可溶性和不溶性部分进行免疫印迹分析。用125I标记的蛋白A检测结合的免疫球蛋白。
20例（74%）近期发病的RA患者血清中的抗体以疾病和组织特异性及非特异性方式识别5种抗原中的至少1种。在2份血清中检测到通常与原发性干燥综合征相关的抗La抗体。8份血清对IgG重链和轻链二聚体的反应性增强。在RA和正常滑膜裂解物（不溶性部分）中，8份血清与35-kd双条带、3份血清与55-kd条带存在强结合。2份血清仅在RA滑膜裂解物（可溶性部分）中独特地识别一种45-kd蛋白。
近期发病的RA患者血清中的IgG抗体对35-kd、55-kd和45-kd这3种新的滑膜抗原以及2种先前已鉴定的抗原（La和IgG）呈现阳性免疫印迹。因此，即使在RA临床病程早期，多种滑膜抗原似乎也能被B细胞识别。
