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巴氏甲烷八叠球菌裂解物对乙酸盐的产甲烷裂解作用

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri.

作者信息

Baresi L

出版信息

J Bacteriol. 1984 Oct;160(1):365-70. doi: 10.1128/jb.160.1.365-370.1984.

DOI:10.1128/jb.160.1.365-370.1984
PMID:6480559
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214726/
Abstract

Cell lysates of acetate-grown Methanosarcina barkeri 227 were found to cleave acetate to CH4 and CO2. The aceticlastic reaction was identified by using radioactive methyl-labeled acetate. Cell lysates decarboxylated acetate in a nitrogen atmosphere, conserving the methyl group in methane. The rate of methanogenesis from acetate in the cell lysates was comparable to that observed with whole cells. Aceticlastic activity was found in the particulate fraction seperate from methylcoenzyme M methylreductase activity, which occurs in the soluble fraction. Pronase treatment eliminated methylcoenzyme M methylreductase activity in lysates and stimulated aceticlastic activity, indicating the aceticlastic activity was not derived from unbroken cells, which are unaffected by proteolytic treatment.

摘要

研究发现，以乙酸盐为生长底物的巴氏甲烷八叠球菌227的细胞裂解物可将乙酸盐裂解为甲烷和二氧化碳。通过使用放射性甲基标记的乙酸盐确定了乙酸裂解反应。细胞裂解物在氮气氛围中使乙酸盐脱羧，将甲基保留在甲烷中。细胞裂解物中由乙酸盐产生甲烷的速率与完整细胞的观测速率相当。乙酸裂解活性存在于与甲基辅酶M甲基还原酶活性分离的颗粒部分，而甲基辅酶M甲基还原酶活性存在于可溶部分。蛋白酶处理消除了裂解物中的甲基辅酶M甲基还原酶活性并刺激了乙酸裂解活性，这表明乙酸裂解活性并非来自未破碎的细胞，因为未破碎的细胞不受蛋白水解处理的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74b2/214726/67367d650010/jbacter00227-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74b2/214726/67367d650010/jbacter00227-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/74b2/214726/67367d650010/jbacter00227-0376-a.jpg

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本文引用的文献

1
Studies on the Methane Fermentation: VI. The Influence of Carbon Dioxide Concentration on the Rate of Carbon Dioxide Reduction by Molecular Hydrogen.甲烷发酵研究：VI. 二氧化碳浓度对分子氢还原二氧化碳速率的影响。
Proc Natl Acad Sci U S A. 1943 Jun;29(6):184-90. doi: 10.1073/pnas.29.6.184.
2
Effect of H(2)-CO(2) on Methanogenesis from Acetate or Methanol in Methanosarcina spp.H(2)-CO(2) 对产甲烷菌利用乙酸或甲醇产甲烷的影响。
Appl Environ Microbiol. 1983 Aug;46(2):348-55. doi: 10.1128/aem.46.2.348-355.1983.
3
Anaerobic Degradation of Lactate by Syntrophic Associations of Methanosarcina barkeri and Desulfovibrio Species and Effect of H(2) on Acetate Degradation.
无法产生甲烷或从乙酸盐中同化碳的甲烷八叠球菌突变体。
J Bacteriol. 1985 Nov;164(2):618-25. doi: 10.1128/jb.164.2.618-625.1985.
4
Methanogenesis and ATP synthesis in a protoplast system of Methanobacterium thermoautotrophicum.嗜热自养甲烷杆菌原生质体系统中的甲烷生成与ATP合成
J Bacteriol. 1986 Nov;168(2):892-900. doi: 10.1128/jb.168.2.892-900.1986.
5
Bioenergetics of methanogenesis from acetate by Methanosarcina barkeri.巴氏甲烷八叠球菌利用乙酸盐进行甲烷生成的生物能量学
J Bacteriol. 1988 Mar;170(3):1369-72. doi: 10.1128/jb.170.3.1369-1372.1988.
6
Methanogens and the diversity of archaebacteria.产甲烷菌与古细菌的多样性
Microbiol Rev. 1987 Mar;51(1):135-77. doi: 10.1128/mr.51.1.135-177.1987.
7
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J Bacteriol. 1986 Dec;168(3):1053-8. doi: 10.1128/jb.168.3.1053-1058.1986.
产甲烷八叠球菌与脱硫弧菌属种的协同作用对乳酸的厌氧降解及 H(2)对乙酸降解的影响。
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5
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6
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J Bacteriol. 1951 Jan;61(1):81-6. doi: 10.1128/jb.61.1.81-86.1951.
7
The methane fermentations of acetate and methanol.乙酸盐和甲醇的甲烷发酵
J Bacteriol. 1957 Jun;73(6):736-42. doi: 10.1128/jb.73.6.736-742.1957.
8
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J Bacteriol. 1982 Jan;149(1):247-54. doi: 10.1128/jb.149.1.247-254.1982.
9
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10
Acetate, methanol and carbon dioxide as substrates for growth of Methanosarcina barkeri.乙酸盐、甲醇和二氧化碳作为巴氏甲烷八叠球菌生长的底物。
Antonie Van Leeuwenhoek. 1980;46(6):601-10. doi: 10.1007/BF00394016.