ATP诱导的体外组装微管凝胶化收缩
ATP-induced gelation--contraction of microtubules assembled in vitro.
作者信息
Weisenberg R C, Cianci C
出版信息
J Cell Biol. 1984 Oct;99(4 Pt 1):1527-33. doi: 10.1083/jcb.99.4.1527.
Abstract
We report here an ATP-dependent formation and contraction, or syneresis, of a microtubule gel using microtubule proteins prepared from calf brains. Gel contraction is typically observable 15-30 min after ATP addition to microtubules assembled to steady state, and is complete after approximately 60 min, at which time the gel volume is reduced by as much as 75%. In contracted gels, microtubule bundles and aster-like structures are observable. Gelation-contraction requires only microtubule proteins present after purification by three cycles of assembly and disassembly.
摘要
我们在此报告，使用从小牛脑制备的微管蛋白可形成一种依赖ATP的微管凝胶并使其收缩，即脱水收缩。在将ATP添加到组装至稳态的微管后，通常在15 - 30分钟后可观察到凝胶收缩，约60分钟后收缩完成，此时凝胶体积减少多达75%。在收缩的凝胶中，可观察到微管束和星状结构。凝胶化收缩仅需要经过三轮组装和拆卸纯化后存在的微管蛋白。
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本文引用的文献
1
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Nature. 1981 Feb 26;289(5800):813-4. doi: 10.1038/289813a0.
2
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J Mol Biol. 1980 Jun 5;139(4):641-59. doi: 10.1016/0022-2836(80)90053-4.
3
Inhibition of microtubule assembly by phosphorylation of microtubule-associated proteins.微管相关蛋白磷酸化对微管组装的抑制作用。
Biochemistry. 1980 May 27;19(11):2472-9. doi: 10.1021/bi00552a027.
4
Purification and assay of microtubule-associated proteins (MAPs).微管相关蛋白（MAPs）的纯化与测定。
Methods Enzymol. 1982;85 Pt B:409-16. doi: 10.1016/0076-6879(82)85041-6.
5
ATP-induced formation of an associated complex between microtubules and neurofilaments.三磷酸腺苷（ATP）诱导微管与神经丝之间形成相关复合物。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1431-5. doi: 10.1073/pnas.78.3.1431.
6
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